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| Name | Sodium nitroprusside Test |
| Video Uploader | Video From CHAMELI DEVI INSTITUTE OF PHARMACY INDORE |
| Upload Date | This Video Uploaded At 22-01-2021 09:35:10 |
| Video Discription | Qualitative test of proteins / amino acid Sodium nitroprusside Test Nitroprusside test is a biochemical test used for the detection of the free –SH groups in amino acids or the cysteine amino acid in a protein. The test is a specific test for cysteine as it is the only amino acid with a free –SH group in the structure. The test, however, is not specific to proteins and amino acids as it can detect compounds like ketones. The test is a colored reaction of amino acid where the free –SH group in the amino acid reacts with the nitroprusside reagent to form a red-colored complex. Cysteine and methionine are two amino acids with –SH groups but the –SH group in the methionine molecule is involved in the thioester linkage which is difficult to break and thus, cannot be detected by this test. The test can also be used for the differentiation between cysteine and cystine as cysteine gives a positive result to this test, but cystine doesn’t. The test is based on the detection of free sulfur atoms in a solution as a result of the degradation of the –SH group. Nitroprusside is a complex anion consisting of an octahedral ferrous center which is surrounded by five tightly bound cyanide groups. The molecule, when combined with a sulfur atom forms a violet or red-colored complex. The action of a strong alkali releases SH group foud in cysteine molecule. The sulfur then reacts with the nitroprusside ion to form a red-colored compound. Some proteins might not give a positive result immediately, and in that case, the solution should be heated as these proteins might require heat coagulation to release the –SH group. |
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